Regulatory

Part:BBa_K619889:Design

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-26)

prfA-UTR, RNA thermosensor from Listeria monocytogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region.

Source

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.

References

Johansson J. et al., An RNA Thermosensor Controls Expression of Virulence Genes in Listeria Monocytogenes. (2001) Cell Vol. 110, 551-561.