Regulatory
Part:BBa_K619889:Design
Designed by: Julianne Grose Group: iGEM11_BYU_Provo (2011-09-26)
prfA-UTR, RNA thermosensor from Listeria monocytogenes
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 18
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region.
Source
The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.